Descriptions of Cell Line Metadata
- Scott Saccone
Contents
General Metadata
The table below describes some of the fields found in the display of iPSCs and Fibroblasts in the catalog. Note that when the value of a field is blank for a given line that field is not displayed. For example, here is a line where only a subset of the possible fields are displayed; only the non-blank fields are shown.
Note that the term sister clone refers to one of a group of clones, such as for iPSCs, generated from a single fibroblast cell line (FCL).
| Field | Description | Values |
|---|---|---|
| Alternative Lines | Lines that may be used as alternatives for removed lines. These are chosen to have similar properties to the removed lines. | Text |
| Cell Line ID | This is the "ND" NINDS Catalog ID shown at the top, it is not labeled | "ND" + 5-digit numeric ID |
| Clone ID | ID (typically RUCDR) of individual clone picked from reprogramming of subject | 9-digit alphanumeric ID |
| Comprehensive QC Clone | Yes/No flag for sister clone with the most comprehensive QC package | Yes/No |
| Edited | This is an edited cell line | Yes/No |
| Editing Method | The editing method, such as CRISPR/Cas9 | Text |
| Edit Location | The mutation relevant to the editing process | Text |
| Gene Edited | The gene name, when applicable, for the editing mutation | Text |
BID of Isogenic Pair | The biomaterial ID for the other line in the editing process | "BID" + 5-digit numeric ID |
| BID of Source iPSC | The biomaterial ID for the source iPSC used in the editing process | "BID" + 5-digit numeric ID |
| Freeze Method | Media in which cells are frozen | Text |
| GMP Grade | Quality of line. See our FAQ for more information on GMP quality lines. | Research, GMP |
| Last Updated | When the data for the line was last updated in the catalog | Date |
| Main Clone ID | Catalog ID of the sister clone with the most comprehensive QC | 9-digit alphanumeric ID |
| Passage at Freeze | Passage at time of freeze | Text |
| Percent CO2 | CO2 percentage during incubation | Percentage |
| Percent O2 | O2 percentage during incubation | Percentage |
| Reason Removed | Why the line was removed from the catalog | Text |
| Removed | Whether the line has been removed from the catalog | Yes/No |
| Replaces Line | The line this line replaces | "ND" + 5-digit numeric ID |
| RUID | The internal RUCDR subject ID which is shown on the vials | 9-digit alphanumeric ID |
| Serum | Percentage of serum in the media | May be descriptive text rather than numeric |
| Source Cell | Source cell used for iPSC derivation | Text |
| Source FCL | ID of Fibroblast used to derive cell line. When the line is not in the NINDS Catalog, a link is provided to an external source. | "ND" + 5-digit numeric ID |
| Split Ratio | Recommended plating ratio at time of passage | Text |
| Standard QC Clone | Yes/No flag for sister clone with the standard QC package | Yes/No |
| Substrate | Plate matrix | Text |
| Temperature | Temperature during incubation | Temperature, possibly with F/C indicator |
| Reprogramming Vector | Method for delivering reprogramming transcription factors | Text |
QC+ icons
Some lines are designated as Comprehensive QC Clones. As described in the table above, this means the clone has the most comprehensive QC package. Such lines are highlighted with a special QC+ icon like this one for subject NDS00010:
GMP Grade Lines
Lines in the GMP Project can either be of Research grade or GMP grade. GMP grade lines require a special ordering process. Special icons indicate Research and GMP lines, as shown for subject NDS00263:
Please contact us if you have questions.
