Descriptions of Cell Line Metadata
Contents
General Metadata
The table below describes some of the fields found in the display of iPSCs and Fibroblasts in the catalog. Note that when the value of a field is blank for a given line that field is not displayed. For example, here is a line where only a subset of the possible fields are displayed; only the non-blank fields are shown.
Note that the term sister clone refers to one of a group of clones, such as for iPSCs, generated from a single fibroblast cell line (FCL).
| Field | Description | Values |
|---|---|---|
| Alternative Lines | Lines that may be used as alternatives for removed lines. These are chosen to have similar properties to the removed lines. | Text |
| Cell Line ID | This is the "ND" NINDS Catalog ID shown at the top, it is not labeled | "ND" + 5-digit numeric ID |
| Clone ID | ID (typically RUCDR) of individual clone picked from reprogramming of subject | 9-digit alphanumeric ID |
| Comprehensive QC Clone | Yes/No flag for sister clone with the most comprehensive QC package | Yes/No |
| Edited | This is an edited cell line | Yes/No |
| Editing Method | The editing method, such as CRISPR/Cas9 | Text |
| Edit Location | The mutation relevant to the editing process | Text |
| Gene Edited | The gene name, when applicable, for the editing mutation | Text |
BID of Isogenic Pair | The biomaterial ID for the other line in the editing process | "BID" + 5-digit numeric ID |
| BID of Source iPSC | The biomaterial ID for the source iPSC used in the editing process | "BID" + 5-digit numeric ID |
| Freeze Method | Media in which cells are frozen | Text |
| GMP Grade | Quality of line. See our FAQ for more information on GMP quality lines. | Research, GMP |
| Last Updated | When the data for the line was last updated in the catalog | Date |
| Main Clone ID | Catalog ID of the sister clone with the most comprehensive QC | 9-digit alphanumeric ID |
| Passage at Freeze | Passage at time of freeze | Text |
| Percent CO2 | CO2 percentage during incubation | Percentage |
| Percent O2 | O2 percentage during incubation | Percentage |
| Reason Removed | Why the line was removed from the catalog | Text |
| Removed | Whether the line has been removed from the catalog | Yes/No |
| Replaces Line | The line this line replaces | "ND" + 5-digit numeric ID |
| RUID | The internal RUCDR subject ID which is shown on the vials | 9-digit alphanumeric ID |
| Serum | Percentage of serum in the media | May be descriptive text rather than numeric |
| Source Cell | Source cell used for iPSC derivation | Text |
| Source FCL | ID of Fibroblast used to derive cell line. When the line is not in the NINDS Catalog, a link is provided to an external source. | "ND" + 5-digit numeric ID |
| Split Ratio | Recommended plating ratio at time of passage | Text |
| Standard QC Clone | Yes/No flag for sister clone with the standard QC package | Yes/No |
| Substrate | Plate matrix | Text |
| Temperature | Temperature during incubation | Temperature, possibly with F/C indicator |
| Reprogramming Vector | Method for delivering reprogramming transcription factors | Text |
QC+ icons
Some lines are designated as Comprehensive QC Clones. As described in the table above, this means the clone has the most comprehensive QC package. Such lines are highlighted with a special QC+ icon like this one for subject NDS00010:
GMP Grade Lines
Lines in the GMP Project can either be of Research grade or GMP grade. GMP grade lines require a special ordering process. Special icons indicate Research and GMP lines, as shown for subject NDS00263:
Please contact us if you have questions.
